Journal of Biotechnology

ISSN: 0168-1656

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Potential of microalgae as a sustainable feed ingredient for aquaculture

Senthil Nagappan, Probir Das and 7 more Open Access November 2021

Application of next generation sequencing in clinical microbiology and infection prevention

Ruud H. Deurenberg, Erik Bathoorn and 11 more Open Access February 2017

Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection

Xiquan Liang, Jason Potter and 10 more Open Access August 2015

Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA

Xiquan Liang, Jason Potter and 3 more Open Access January 2017

Bacterial enzymes involved in lignin degradation

Gonzalo de Gonzalo, Dana I. Colpa, Mohamed H.M. Habib, Marco W. Fraaije Open Access October 2016

Polyhydroxyalkanoate bio-production and its rise as biomaterial of the future

Tania Palmeiro-Sánchez, Vincent O’Flaherty, Piet N.L. Lens Open Access March 2022

Impacts of fast production of afucosylated antibodies and Fc mutants in ExpiCHO-S™ for enhancing FcγRIIIa binding and NK cell activation

Xiaotian Zhong, Jennifer Schenk and 24 more Open Access December 2022

In vitro models of medulloblastoma: Choosing the right tool for the job

Delyan P. Ivanov, Beth Coyle, David A. Walker, Anna M. Grabowska Open Access October 2016

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

Astrid Weninger, Anna-Maria Hatzl and 3 more Open Access October 2016

Therapeutic glycoprotein production in mammalian cells

Marie-Eve Lalonde, Yves Durocher Open Access June 2017

The use of bacteriophages to biocontrol oral biofilms

Szymon P. Szafrański, Andreas Winkel, Meike Stiesch Open Access May 2017

Biological control of plant pathogens by Bacillus species

Djordje Fira, Ivica Dimkić and 3 more November 2018

Strategies for analyzing bisulfite sequencing data

Katarzyna Wreczycka, Alexander Gosdschan and 4 more Open Access November 2017

Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand

Stefan Kittler, Julian Ebner and 9 more Open Access November 2022

lac operon induction in Escherichia coli: Systematic comparison of IPTG and TMG induction and influence of the transacetylase LacA

Anja Marbach, Katja Bettenbrock January 2012

Towards maximum acceleration of monoclonal antibody development: Leveraging transposase-mediated cell line generation to enable GMP manufacturing within 3 months using a stable pool

Valerie Schmieder, Juergen Fieder and 21 more Open Access April 2022

Small-scale GMP production of plasmid DNA using a simplified and fully disposable production method

Noor A.M. Bakker, Renate de Boer and 6 more Open Access December 2019

Systematic analysis of CRISPR–Cas9 mismatch tolerance reveals low levels of off-target activity

Emily M. Anderson, Amanda Haupt and 9 more Open Access October 2015

Extraction of astaxanthin from microalga Haematococcus pluvialis in red phase by using generally recognized as safe solvents and accelerated extraction

Antonio Molino, Juri Rimauro and 9 more Open Access October 2018

Recent advances in CRISPR/Cas9-mediated knock-ins in mammalian cells

Mehdi Banan January 2020

Advanced genetic strategies for recombinant protein expression in Escherichia coli

Hans Peter Sørensen, Kim Kusk Mortensen January 2005

Advanced PHB fermentation strategies with CO2-derived organic acids

Elodie Vlaeminck, Koen Quataert and 3 more Open Access January 2022

Flashing lights affect the photophysiology and expression of carotenoid and lipid synthesis genes in Nannochloropsis gaditana

Serena Lima, Jep Lokesh and 7 more Open Access December 2022

Environmental analysis of plastic production processes: Comparing petroleum-based polypropylene and polyethylene with biologically-based poly-β-hydroxybutyric acid using life cycle analysis

K.G. Harding, J.S. Dennis, H. von Blottnitz, S.T.L. Harrison May 2007

Bioinformatics for NGS-based metagenomics and the application to biogas research

Sebastian Jünemann, Nils Kleinbölting and 10 more Open Access November 2017 no longer supports Internet Explorer.

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Biotechnology research, development, applications and management in Zimbabwe: Review

Profile image of Wilson Parawira

Information on the nature, trend and quality of biotechnology research, development, applications and management in a country is generally needed to improve policy decisions, guide future research and cooperation, civil society and general public knowledge, technology development agencies and to support public sector implementation of biotechnology research. However, there has been to date no comprehensive publication describing overall biotechnology research and development in Zimbabwe. The little information available only cover agricultural biotechnology in particular plant biotechnology. This paper, therefore, offers a profile of Zimbabwe's overall biotechnology research and development covering activities that provide new knowledge, and the application of biotechnological techniques, biosafety and the regulation status of the biotechnology in the country. The aim is to provide an insight and update of the state of biotechnology in Zimbabwe. This paper also aims to highlight the...

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This paper aims at valorising an artisanal honey based alcoholic beverage from northern Cameroon (Africa). The studied process production involved most of stage required for production of mead with difference in starter preparation and handling which include a step of drying and smoking. The must preparation also present light difference with the one of wine production as there’s no activator neither extra nutrients added. But a step of preparation of a so called “Mbii dong” is necessary. “Mbii dong” is a mixture of diluted honey with hot pepper or sometime spices. Some of the accessed physicochemical parameters which include pH, total titrable acidity, residual sugar and total polyphenols indicate similarities with that of mead. It also has to highlight the relatively high alcohol (around 15.4±0.6%) content of the beverage. The recorded values of physicochemical components as alcoholic, volatile acidity, polyphenols… may confirm the fact that a part of festivities, “kuri” can be used as medicines.

James Owusu-Kwarteng

Fura is a spontaneously fermented pearl millet product consumed inWest Africa. The yeast species involved in the fermentation were identified by pheno- and genotypic methods to be Candida krusei, Kluyveromyces marxianus, Candida tropicalis, Candida rugosa, Candida fabianii, Candida norvegensis and Trichosporon asahii. C. krusei and K. marxianus were found to be the dominant species. Survival in pH 2.5 or in the presence of bile salts (0.3% (w/v) oxgall) and growth at 37 °C were independently determined as indicators of the survival potential of the isolates during passage through the human gastrointestinal tract. Selected yeast species isolates were assessed for their probiotic potential. All of the examined yeast isolates survived and grewat human gastrointestinal conditions in pH 2.5, 0.3% (w/v) oxgall at 37 °C. The effect on the transepithelial electrical resistance (TEER) across polarized monolayers of intestinal epithelial cells of human (Caco-2) and porcine (IPEC-J2) origin, were determined. The Caco-2 cells and IPEC-J2 cells displayed clearly different relative TEER results. The strains of C. krusei, K. marxianus, C. rugosa and T. asahii were able to increase the relative TEER of Caco-2 monolayers after 48 h. In comparison, the relative TEER of IPEC-J2 monolayers decreased when exposed to the same yeasts, even though T. asahii did not differ significantly from Saccharomyces cerevisiae var. boulardii which is used as a human probiotic. C. tropicalis resulted in the largest relative TEER decrease for both the human and the porcine cell model assays. Hyphal growth was observed for C. albicans and C. tropicalis after 48 h of incubation with polarized Caco-2 monolayers, whereas this was not the case for the remaining yeast species. In the present study new yeast strains with potential probiotic properties have been isolated to be used potentially as starter cultures for fura production.

Bennie Viljoen

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Home > Chemical and Biomolecular Engineering > Research Papers & Publications > Biotechnology

Chemical and Biomolecular Engineering Research and Publications

Papers in biotechnology.

In vitro evaluation of PEGylated mesoporous MgFe2O4 magnetic nanoassemblies (MMNs) for chemo-thermal therapy , Sunil Kumar, Amita Daverey, Niroj Kumar Sahu, and Dhirendra Bahadur

Identification and characterization of calcium and manganese transporting ATPase (PMR1) gene of Pichia pastoris , Michael P. Dux and Mehmet Inan

A Comparative Study of Monoclonal Antibodies (Mabs) Purified from Cell Culture Supernatant on EDTPA-Modified Zirconia Beads and Protein A-Hyper D Support , Anuradha Subramanian, Blanca Martinez, Jill Holm, Peter W. Carr, and Clayton V. McNeff

Use of Modified Chitosan Macrospheres in the Selective Removal of Immunoglobulins , Anuradha Subramanian, Swapan Roy, Carmine Mascoli, and Jennifer Hommerding

Use of a modified zirconia support in the separation of immunoproteins , Anuradha Subramanian and Sabyasachi Sarkar

UV-vis -infrared optical and AFM study of spin-cast chitosan films , W H. Nosal, D. W. Thompson, Sabyasachi Sarkar, Anuradha Subramanian, John A. Woollam, and L Yan

Identification of the Mass transfer mechanisms involved in the transport of human immunoglobulin - G in N,N,N',N' -ethylnediaminetetramenthylenephosphonic acid modified Zirconia , Sabyasachi Sarkar, Perer W. Carr, and Anuradha Subramanian

Modeling of immunoglobulin uptake by N,N,N′,N′-ethylenediaminetetramethylenephosphonic acid-modified zirconia particles under static and dynamic conditions , Sabyasachi Sarkar and Anuradha Subramanian

Chromatographic Purification of MAbs with Non-Affinity Supports , Anuradha Subramanian

The use of confocal laser scanning microscopy to study the transport of biomacromolecules in a macroporous support , Anuradha Subramanian and Jennifer Hommerding

Crosslinked chitosan: Its physical properties and the effects of matrix stiffness on chondrocyte cell morphology and proliferation , Anuradha Subramanian and Hsin-YI Lin

Preparation and evaluation of the electrospun chitosan/PEO fibers for potential applications in cartilage tissue engineering , Anuradha Subramanian, David Vu, Gustavo F. Larsen, and Hsin YIi Lin

Secretion of Recombinant Human fibrinogen by the Murine Mammary Gland , William H. Velander

Nonequilibrium Thermodynamics in Engineering and Science , Y. Demirel and Stanley I. Sandler

Production and purification of a chimeric monoclonal antibody against botulinum neurotoxin serotype A , Mark C. Mowry, Michael Meagher LEAD INVESTIGATOR, Leonard Smith, and Anuradha Subramanian

Causes of Proteolytic Degradation of Secreted Recombinant Proteins Produced in ethylotrophic Yeast Pichia pastoris: Case Study With Recombinant Ovine Interferon-T , Jayanta Sinha, Bradley A. Plantz, Mehmet Inan, and Michael Meagher

Haemophilic Factors Produced by Transgenic Livestock: Abundance that can enable alternative therapies worldwide , Kevin E. Van Cott, Paul E. Monahan, Timothy C. Nichols, and William H. Velander

Transgenic non-human mammals expressing human coagulation factor VIII and von Willebrand factor , Henryk Lubon, William N. Drohan, and William H. Velander

Characterization and optimization of a chromatographic process based on ethylenediamine-N,N,N′,N′-tetra(methylphosphonic) acid-modified zirconia particles , Sabyasachi Sarkar, Peter W. Carr, Clayton V. McNeff, and Anuradha Subramanian

An approach to sequence DNA without tagging , Sanjun Niu and Ravi F. Saraf

Expression of active human factor IX in mammary tissue and of milk non human transgenic mammals , William H. Velander and William N. Drohan

Expression of a heterologous protein C in mammary tissue of transgenic animals using a long whey acidic protein promoter , William H. Velander, Henryk Lubton, William N. Drohan, and Lothar Hennighausen

Immunoaffinity Chomatography , Anuradha Subramanian

Purification of immunoglobulins from Serum Using Thiophilic Cellulose Beads , Anuradha Subramanian

Ranking the Factors Impacting lmmunosorbent Performance , Anuradha Subramanian and William H. Velander

Modeling Pichia pastoris Growth on Methanol and Optimizing the Production of a Recombinant Protein, the Heavy-Chain Fragment C of Botulinum Neurotoxin, Serotype A , Wenhui Zhang, Mark A. Bevins, Bradley A. Plantz, Leonard A. Smith, and Michael M. Meagher

Bovine Follicular Dynamics, Oocyte Recovery,and Development of Oocytes Microinjected with a Green Fluorescent Protein Construct , M S. CHAUHAN, S Nadir, T L. Bailey, A W. Pryor, S P. Butler, D R. Notter, William H. Velander, and F C. Gwazdauskas

A Red-Dot-Blot Protein Assay Technique in the Low Nanogram Range , Tiilin Marcol and Anuradha Subramanian

Bioactivities of a tumour necrosis like factor released by chicken macrophages , Silke Rautenschlein, Anuradha Subramanian, and Jagdev M. Sharma

Bioactivities of a tumour necrosis like factor released by chicken rnacrophages , Silke Rautenschlein, Anuradha Subramanian, and Jagdev M. Sharma

Crossflow Microfiltration of Recombinant Escherichia coli Lysates after High Pressure Homogenization , Stuart M. Bailey and Michael M. Meagher

Effect of Antibody Orientation on Immunosorbent , Anuradha Subramanian and William H. Velander

Affinity Purification of Biologically Active andInactive Forms of Recombinant Human Protein C Produced in Porcine Mammary Gland , Kevin E. Van Cott; Barry Williams Department of Chemical Engineering,Virginia Tech University, Blacksburg, VA 24061, USA; William H. Velander; Francis C. Gwazdauskas; Tim Lee Jerome Holland Laboratory, American Red Cross, Rockville, MD 20855, USA; Henryk Lubon; and William N. Drohan

Expression of a functional human complement inhibitor in a transgenic pig as a model for the prevention of xenogeneic hyperacute organ rejection. , William L. Fodor, Barry L. Williams, Louis A. MArtis, Josaeph A. Madri, Scott A. Rollins, James W. Knight, William H. Velander, and Stephen P. Squinto

Effect of Culture Conditions, Donor Age, and Injection Site on In Vitro Development of DNA Microinjected Porcine Zygotes , M A. Hajdu, J W. Knight, R S. Cansecot, R L. Krisher, William H. Velander, R E. Pearson, and F C. Gwazdauskas

Effects of Time of Deoxyribonucleic Acid Microinjection on Gene Detection and In Vitro Development of Bovine Embryos , A.E T. Sparks, R S. Canseco, C G. Russell, J L. Johnson, H D. Moll, William H. Velander, and Francis C. Gwazdauskas

High-level expression of a heterologous protein in the milk of transgenic swine using the cDNA encoding human protein C , William H. Velander, John L. Johnson, Raymond L. Page, Christopher G. Russell, Anuradha Subramanian, Tracy D. Wilkins, Francis C. Gwazdauskas, Christoph Pittius, and William N. Drohan

High-level expression of a heterologous protein in the milk of transgenic swine using the cDNA encoding human protein C , William H. Velander, John L. Johnson, Raymond L. Page, Chritopher G. Russell, Anuradha Subramanian, Tracy D. Wilkins, Francis C. Gwazdauskas, Christoph Pittus, and William N. Drohan

In Vitro Development of Zygotes from Prepubertal Gilts After Microinjection of DNA , B. L. Williams, J. L. Johnson, A. E.T. Sparks, R. S. Canseco, J. W. Knight, William H. Velander, R. L. Page, W. N. Drohan, J. M. Young, R. E. Pearson, T. D. Wilkins, and F. C. Gwazdauskas

Enzyme- Facilitated Transport and Separation of Organic Acids through Liquid Membranes , David C. Rethwisch, Anuradha Subramanian, Gao Yi, and Jonathan S. Dordick

Conformational Changes in an Epitope Localized to the NH2-terminal Region of Protein C , Carolyn L. Orthener, Rapti D. Madurawe, William H. Velander, William N. Drohan, Frances D. Bettey, and Dudley K. Strickhand

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Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein

Mammalian cell lines are frequently used as protein expression hosts because of their ability to correctly fold and assemble complex proteins, produce them at high titers, and confer post-translational modific...

A fully human connective tissue growth factor blocking monoclonal antibody ameliorates experimental rheumatoid arthritis through inhibiting angiogenesis

Connective tissue growth factor (CTGF) plays a pivotal role in the pathogenesis of rheumatoid arthritis (RA) by facilitating angiogenesis and is a promising therapeutic target for RA treatment. Herein, we gene...

Black glucose-releasing silicon elastomer rings for fed-batch operation allow measurement of the oxygen transfer rate from the top and optical signals from the bottom for each well of a microtiter plate

In industrial microbial biotechnology, fed-batch processes are frequently used to avoid undesirable biological phenomena, such as substrate inhibition or overflow metabolism. For targeted process development, ...

In vitro culture of bovine fibroblasts using select serum-free media supplemented with Chlorella vulgaris extract

Standard cell culture practices require the addition of animal-derived serum to culture media to achieve adequate cell growth. Typically, 5–10% by volume of fetal bovine serum (FBS) is used, which accounts for...

Biochemical characterization and insights into the potency of the acidic Aspergillus niger NRC114 purified α-galactosidase in removing raffinose family oligosaccharides from soymilk yogurt

Because humans lack α-galactosidase, foods containing certain oligosaccharides from the raffinose family, such as soybeans and other legumes, may disrupt digestion and cause flatulence.

Production of rhamnolipid biosurfactants in solid-state fermentation: process optimization and characterization studies

Rhamnolipids are a group of the extracellular microbial surface-active molecules produced by certain Pseudomonas species with various environmental and industrial applications. The goal of the present research wa...

Efficient expression of fusion human epidermal growth factor in tobacco chloroplasts

Chloroplast transformation is a robust technology for the expression of recombinant proteins. Various types of pharmaceutical proteins including growth factors have been reported in chloroplasts via chloroplas...

Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design

Docosahexaenoic acid (DHA) can improve human and animal health, particularly including anti-inflammatory, antioxidant, anticancer, neurological, and visual functions. Schizochytrium sp. is a marine heterotrophic ...

Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene

Newcastle disease virus (NDV) strain ZM10, a typical enterotropic avirulent vaccine strain, has been widely used in China for chickens against Newcastle disease. To elucidate its enterotropic mechanism and dev...

Correction: Identifcation, expression, and purifcation of DNA cytosine 5-methyltransferases with short recognition sequences

The original article was published in BMC Biotechnology 2022 22 :33

Development of a microbial protease for composting swine carcasses, optimization of its production and elucidation of its catalytic hydrolysis mechanism

Dead swine carcass composting is an excellent method for harmless treatment and resource utilization of swine carcass. However, poor biodegradation ability of traditional composting results in poor harmless tr...

Toxic effects of trace phenol/guanidine isothiocyanate (P/GI) on cells cultured nearby in covered 96-well plates

A mixture of phenol and guanidine isothiocyanate (“P/GI”, the principal components of TRIzol™ and similar products) is routinely used to isolate RNA, DNA, and proteins from a single specimen. In time-course ex...

Modeling and optimizing in vitro percentage and speed callus induction of carrot via Multilayer Perceptron-Single point discrete GA and radial basis function

Callus induction is the first step in optimizing plant regeneration. Fit embryogenesis and shooting rely on callus induction. In addition, using artificial intelligence models in combination with an algorithm ...

Identification, expression, and purification of DNA cytosine 5-methyltransferases with short recognition sequences

DNA methyltransferases (MTases) are enzymes that induce methylation, one of the representative epigenetic modifications of DNA, and are also useful tools for analyzing epigenomes. However, regarding DNA cytosi...

The Correction to this article has been published in BMC Biotechnology 2022 22 :37

Preparation of a novel monoclonal antibody against active components of PHA-L from Phaseolus vulgaris and its functional characteristics

Leukocyte phytohemagglutinin (PHA-L), derived from the L4 tetramer of PHA, has been frequently employed as a mitogen to induce T lymphocyte proliferation in vitro. The biological application of PHA-L in cancer...

Identification of a novel fully human anti-toxic shock syndrome toxin (TSST)-1 single-chain variable fragment antibody averting TSST-1-induced mitogenesis and cytokine secretion

Staphylococcal superantigens are virulence factors that help the pathogen escape the immune system and develop an infection. Toxic shock syndrome toxin (TSST)-1 is one of the most studied superantigens whose r...

Gene cloning and molecular characterization of a thermostable chitosanase from Bacillus cereus TY24

An important conceptual advance in health and the environment has been recognized that enzymes play a key role in the green processing industries. Of particular interest, chitosanase is beneficial for recyclin...

Investigation of neomycin biodegradation conditions using ericoid mycorrhizal and white rot fungal species

In the search for methods to biodegrade recalcitrant compounds, the use of saprotrophic fungi and white rot fungi, in particular belonging to the phylum Basidiomycota, has gained interest. This group of fungi ...

Automatic identification of benign pigmented skin lesions from clinical images using deep convolutional neural network

We aimed to develop a computer-aided detection (CAD) system for accurate identification of benign pigmented skin lesions (PSLs) from images captured using a digital camera or a smart phone.

A new method for screening acute/chronic lymphocytic leukemia: dual-label time-resolved fluorescence immunoassay

Lymphocytic leukemia (LL) is a primary malignant tumor of hematopoietic tissue, which seriously affects the health of children and the elderly. The study aims to establish a new detection method for screening ...

Production of surfactant-stable keratinase from Bacillus cereus YQ15 and its application as detergent additive

With the growing concern for the environment, there are trends that bio-utilization of keratinous waste by keratinases could ease the heavy burden of keratinous waste from the poultry processing and leather in...

Efficient gene delivery into the embryonic chicken brain using neuron-specific promoters and in ovo electroporation

The chicken in ovo model is an attractive system to explore underlying mechanisms of neural and brain development, and it is important to develop effective genetic modification techniques that permit analyses ...

Metabolic engineering of energycane to hyperaccumulate lipids in vegetative biomass

The metabolic engineering of high-biomass crops for lipid production in their vegetative biomass has recently been proposed as a strategy to elevate energy density and lipid yields for biodiesel production. En...

940 nm diode laser induced differentiation of human adipose derived stem cells to temporomandibular joint disc cells

Temporomandibular disorder (TMD) refers to a group of disorders that affect temporomandibular joint (TMJ) and its associated muscles with very limited treatment options. Stem cell research is emerging as one o...

Development of a human phage display-derived anti-PD-1 scFv antibody: an attractive tool for immune checkpoint therapy

The PD-1 checkpoint pathway plays a major role in tumor immune evasion and the development of the tumor microenvironment. Clinical studies show that therapeutic antibodies blocking the PD-1 pathway can restore...

Generation of a recombinant antibody for sensitive detection of Pseudomonas aeruginosa

Pseudomonas aeruginosa ( P. aeruginosa ) is a major pathogen that causes nosocomial infections and often exhibits antibiotic resistance. Therefore, the development of an accurate method for detecting P. aeruginosa ...

Oleic acid based experimental evolution of Bacillus megaterium yielding an enhanced P450 BM3 variant

Unlike most other P450 cytochrome monooxygenases, CYP102A1 from Bacillus megaterium (BM3) is both soluble and fused to its redox partner forming a single polypeptide chain. Like other monooxygenases, it can catal...

Generation of genome-edited dogs by somatic cell nuclear transfer

Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal...

rocF affects the production of tetramethylpyrazine in fermented soybeans with Bacillus subtilis BJ3-2

Tetramethylpyrazine (TTMP) is a flavoring additive that significantly contributes to the formation of flavor compounds in soybean-based fermented foods. Over recent years, the application of TTMP in the food i...

Development of an efficient veterinary rabies vaccine production process in the avian suspension cell line AGE1.CR.pIX

Mass vaccination of dogs as important rabies reservoir is proposed to most effectively reduce and eliminate rabies also in humans. However, a minimum coverage of 70% needs to be achieved for control of the dis...

Oil–water partition coefficient preparation and detection in the dihydroartemisinin self-emulsifying drug delivery system

The aim of the present study is to increase the solubility of dihydroartemisinin (DHA) using the self-emulsifying drug delivery system (SEDDS).

αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8

Small extracellular vesicles (sEVs) are nanometer-sized membranous particles shed by many types of cells and can transfer a multitude of cargos between cells. Recent studies of sEVs have been focusing on their...

Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci

The discovery of the CRISPR-Cas9 system and its applicability in mammalian embryos has revolutionized the way we generate genetically engineered animal models. To date, models harbouring conditional alleles (i...

Method for quantification of porcine type I interferon activity using luminescence, by direct and indirect means

Type I interferons are widely used in research applications and as biotherapeutics. Current assays used to measure interferon concentrations, such as plaque reduction assays and ELISA, are expensive, technical...

A copper switch for inducing CRISPR/Cas9-based transcriptional activation tightly regulates gene expression in Nicotiana benthamiana

CRISPR-based programmable transcriptional activators (PTAs) are used in plants for rewiring gene networks. Better tuning of their activity in a time and dose-dependent manner should allow precise control of ge...

Isolation and identification of a feather degrading Bacillus tropicus strain Gxun-17 from marine environment and its enzyme characteristics

Feathers are the most abundant agricultural waste produced by poultry farms. The accumulation of a large number of feathers not only seriously pollutes the environment but also causes the waste of protein reso...

A simplified Gibson assembly method for site directed mutagenesis by re-use of standard, and entirely complementary, mutagenesis primers

Site-directed mutagenesis (SDM) is a key method in molecular biology; allowing to modify DNA sequences at single base pair resolution. Although many SDM methods have been developed, methods that increase effic...

Efficient extraction and antioxidant activity of polyphenols from Antrodia cinnamomea

Antrodia cinnamomea , a rare medicinal fungus, has been increasingly studied in recent years because of its abundant secondary metabolites which are beneficial to humans. However, there is a lack of research on it...

Development of a CHO cell line for stable production of recombinant antibodies against human MMP9

Human matrix metalloproteinase 9 (hMMP9) is a biomarker in several diseases, including cancer, and the need for developing detectors and inhibitors of hMMP9 is increasing. As an antibody against hMMP9 can be s...

An efficient and specific CRISPR-Cas9 genome editing system targeting soybean phytoene desaturase genes

Genome editing by CRISPR/Cas9 has become a popular approach to induce targeted mutations for crop trait improvement. Soybean ( Glycine max L. Merr.) is an economically important crop worldwide. Although gene editi...

One-step fermentation for producing xylo-oligosaccharides from wheat bran by recombinant Escherichia coli containing an alkaline xylanase

One-step fermentation is a cheap way to produce xylo-oligosaccharides (XOS), where production of xylanases and XOS is integrated into a single process. In spite of cost advantage, one-step fermentation is stil...

Self-limiting fall armyworm: a new approach in development for sustainable crop protection and resistance management

The fall armyworm, Spodoptera frugiperda , is a significant and widespread pest of maize, sorghum, rice, and other economically important crops. Successful management of this caterpillar pest has historically reli...

Biological modification of pentosans in wheat B starch wastewater and preparation of a composite film

Petrochemical resources are becoming increasingly scarce, and petroleum-based plastic materials adversely impact the environment. Thus, replacement of petroleum-based materials with new and effective renewable...

A study on the processing technology for Rhizoma Coptidis

The present study intends to optimize the processing technology for the wine-processing of Rhizoma Coptidis, using alkaloids as indicators.

Tenebrio molitor : possible source of polystyrene-degrading bacteria

The excessive use of polystyrene as a packaging material has resulted in a rise in environmental pollution. Polystyrene waste has continually increased water pollution, soil pollution and the closing of landfi...

Media optimization for SHuffle T7 Escherichia coli expressing SUMO-Lispro proinsulin by response surface methodology

SHuffle is a suitable Escherichia coli ( E. coli ) strain for high yield cytoplasmic soluble expression of disulfide-bonded proteins such as Insulin due to its oxidative cytoplasmic condition and the ability to cor...

Expression, purification and characterisation of a human anti-CDK4 single-chain variable fragment antibody

Cyclin-dependent kinase 4 (CDK4) when hyperactivated drives development and maintenance of most tumour types, thus prompting its use as an essential cancer treatment target and a diagnostic tool. Target-bindin...

Enzymatic degradation is an effective means to reduce aflatoxin contamination in maize

Aflatoxins are carcinogenic compounds produced by certain species of Aspergillus fungi. The consumption of crops contaminated with this toxin cause serious detrimental health effects, including death, in both liv...

Chloroplast genome sequencing based on genome skimming for identification of Eriobotryae Folium

Whole chloroplast genome (cpDNA) sequence is becoming widely used in the phylogenetic studies of plant and species identification, but in most cases the cpDNA were acquired from silica gel dried fresh leaves. ...

Antibacterial effect of cerium oxide nanoparticle against Pseudomonas aeruginosa

Antibiotics have been widely used for the treatment of bacterial infections for decades. However, the rapid emergence of antibiotic-resistant bacteria has created many problems with a heavy burden for the medi...

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Citation Impact 3.329 -  2-year Impact Factor  (2021) 3.476 -  5-year Impact Factor  (2021) 0.892 - SNIP (Source Normalized Impact per Paper) 0.657 - SJR (SCImago Journal Rank)

Speed 39 days to first decision for all manuscripts (Median) 64 days to first decision for reviewed manuscripts only (Median)

Usage  1,076,751 Downloads (2021) 300 Altmetric mentions (2021)

BMC Biotechnology

ISSN: 1472-6750

Go to journal home page - Current Research in Biotechnology

Current Research in Biotechnology

About the journal.

Excellence paves the way Graphical Reviews: A new way to publish your research

Aims & Scope

Current Research in Biotechnology ( CRBIOT ) is a new primary research, gold open access journal from Elsevier. CRBIOT publishes original papers and short communications (including viewpoints and perspectives ) resulting from research in biotechnology and biotech-associated disciplines. Current …

Article Publishing Charge for open access

* This discount is valid for all authors who wish to publish open access and submit their article by 30 June 2023


Prof. dr. atanas atanasov, phd, dr. habil.

Ludwig Boltzmann Institute for Digital Health and Patient Safety, Wien, Austria

Latest published

Articles in press, most downloaded, most popular, antioxidant capacity and combinatorial antimicrobial effects of nardostachys jatamansi essential oil with conventional antibiotics against some drug resistant bacteria, phenotyping senescent mesenchymal stromal cells using ai image translation, high-sensitivity profiling of glycoproteins from ovarian cancer sera using lectin-affinity and lc-esi-q-tof-ms/ms, antimicrobial peptides with antiviral and anticancer properties and their modification and nanodelivery systems, genetic and metabolic engineering of methanococcus spp, a survey of the biosynthetic potential and specialized metabolites of archaea and understudied bacteria, xylanases from thermophilic archaea: a hidden treasure, high-throughput biointerfaces for direct, label-free, and multiplexed metaplasmonic biosensing, more from current research in biotechnology, announcements, editor spotlight: prof. dr. atanas atanasov, editor collection: impactful research current opinion in biotechnology, special issues and article collections, advanced nanomaterials based biosensors in clinical diagnosis, environmental protection and industrial fermentation, archaea biotechnology, recent advances on bio-based and biodegradable plastics, integration of biological complexity data with artificial intelligence: towards the development of predictive mathematical models, partner journals, current opinion in biotechnology.

Review articles in Current Opinion in Biotechnology are by invitation only. Current Opinion in Biotechnology (COBIOT) publishes authoritative, comprehensive, and systematic reviews. COBIOT helps specialists keep up to date with a clear and readable synthesis on current advances biotechnology. …

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